International Conference on Chronic Fatigue Points to Low-grade Viral Infections in Brain February 22, 2005

 

 

ADDENDUM Dan Peterson, MD

http://www.hhv-6foundation.org/febpressrelease.pdf

Sierra Internal Medicine, Incline Village, Nevada 775-832-0989, ext 201

Dr. Peterson found HHV-6A in 29% of the serum and in 20% of the spinal fluid of his patients with prominent CNS symptoms: neurocognitive problems, headaches, paresthesias or autonomic dysfunction. Of those with virus in the spinal fluid, the blood tests were negative 40% of the time. This means many
physicians get false negative results when they test for HHV-6 in the serum.

Peterson tested 430 patients by PCR or rapid culture, and found 126 were positive at least once. He performed 145 spinal taps on patients with an abnormal MRI or severe problems with cognitive functioning. Peterson found one case each of EBV and CMV and 27 cases of HHV-6, all variant A. He has been treating
them with intravenous antiviral therapy and the majority has responded. Peterson has had success using Ampligen, an antiviral that is currently in clinical trials, and cidofovir, an antiviral approved for CMV retinitis.



Dharam Ablashi, MD
Scientific Director, HHV-6 Foundation - 302-947-9634

Dr. Ablashi reported that when studies used the correct testing method, over 80% of the studies showed a strong association
between HHV-6 and CFS.

When you look only at the studies that tested for active (vs. latent) virus in CFS patients, then 10 out of 12 showed a strong
positive association between HHV-6A infection and CFS", he said. Ablashi found the same to be true in the studies on MS
patients: 29 out of the 37 studies on MS and HHV-6 showed a positive association. Many of the early studies were done on
whole blood, a technique that picks up latent virus, leaving the results muddled. The best tests are those that only pick up active
virus - tests done on plasma, serum or spinal fluid with no cells.
The problem is that although these are the only tests that can indicate active infection, they aren't very sensitive.

There is a good reason why it has taken a long time to build a case for this virus playing a role in chronic fatigue - this is a very
difficult virus to find," said Ablashi. "The virus is 'neurotropic meaning it prefers to live in the brain tissue. "It is quite frequent
that a patient might have a significant infection in the brain tissue, but no virus evident in the serum by PCR."



Takeshi Sairenji, MD
Department of Biomedical Sciences, Tottori University, Tottori,
Japan -  sairen@grape.med.tottori-u.ac.jp

Dr. Sairenji showed evidence that 60% of CFS patients vs. 11% of controls had evidence of chronic activated antiviral pathways.
He suggested that chronic fatigue might be caused by interferon from viral infections such as HHV-6, Epstein Barr Virus and
Borna virus. Sairenji's study supports the previous work of Dr. Kenneth De Meirleir of Brussels and the scientists associated
with RED Laboratories who have found similar distinct physical abnormalities among CFS patients.


Kenneth De Meirleir
Department of Human Physiology, Vrije Universiteit Brussel,
Brussels, Belgium  - de.meirleir@pandora.be


Dr. Kenneth DeMeirleir, who has published similar findings on elevated antiviral pathways, said that these abnormalities result
in the inactivation of the thyroid receptors - so that CFS patients are functionally hypothyroid even though their lab results may
appear normal. He reported that these abnormalities also cause an increased sensitivity to heavy metals such as nickel and
mercury, as well as a sluggish cortisol response.


Kazuyoshi Ikuta, MD
Research Institute for Microbial Diseases, Department of
Virology, Osaka University, Osaka, Japan -
ikuta@biken.osaka-u.ac.jp

Dr. Ikuta reported his group found a higher prevalence of Borna virus antibodies in both psychiatric and CFS patients compared
to controls. He demonstrated that mice injected with Borna virus developed low-grade Borna viral infections in the glial cells of
the brain. This lowgrade infection disrupted neuronal functioncaus the mice to behave abnormally.



Abbijit Chaudhuri, MD
Division of Clinical Neurosciences and Molecular Pathology, University of Glasgow, Scotland, UK - Ac54@udcf.gla.ac.uk

Dr. Chaudhuri, a neurologist from Glasgow, explained that on way that poorly performing glial cells cause fatigue is by altering
ion channels and creating a 'channelopathy.' He believes that there are many triggers for CFS, but they ultimately all end up
with a common pathway in creating fatigue: a disruption of the ion channels.

Chadhuri used as an example, chronic fatigue caused by ciguatera fish poisoning. The ciguatera toxins block the sodium
ion channels, which result in too much extra-cellular potassium, which in turn creates fatigue. Glial cells, which are supposed to
buffer the amount of potassium released from the neurons, do not function well when they are infected with virus. HHV-6A virus
actively reproduces inside the brain's glial cells, while the B variant simply smolders - but both strains induce inflammatory
cytokines.



Kazuhiro Kondo, MD
Chairman of the Department of Microbiology, Jikei University School of Medicine, Tokyo, Japan kkondo@jikei.ac.jp

Dr. Kondo found that 88% of Japanese workers expressed HHV-6 B virus in their saliva when they were stressed just before
the holidays, but found it in only 24% after the holidays when the same workers were refreshed.

There is almost no variant HHV-6 A in Japan so his studies were based on the B variant, which according to Dr. Kondo is far less
pathogenic than the A variant but much quicker to activate.



Norihiro Sadato, MD
Osaka University School of Medicine, Osaka, Japan
sadato@nips.ac.jp

Dr. Sadato used advanced, quantitative MRI techniques to examine 16 CFS patients and 49 controls and found marked
differences. CFS patients had reduced gray-matter volume in the prefrontal cortex and the severity of the atrophy related to the
severity of fatigue in the patients. . Furthermore, when the dysfunctional areas of the brain were pinpointed, they turned out
to be the centers of the brain that control emotion, executive functioning and motivation. Sadato noted that these results were
consistent with previous reports of abnormal acetyl-L-carnitine uptake in the pre-frontal cortex in CFS patients. These
abnormalities are similar to those reported in MS patients, who also have a high incidence of MS.



Benjamin Natelson, MD
Department of Neurosciences, UMDNJ-New Jersey Medical
School, Newark, NJ 973-972-2550

Dr. Natelson, a neurologist who runs a large CFS clinic in New Jersey, was among the speakers presenting evidence of brain
inflammation due to encephalopathy. Natelson found evidence of inflammation in the spinal fluid in 30% of his CFS patients.
Natelson concluded that this study supports the hypothesis that some CFS patients have a brain disease.



Gundrun Lange, MD
Department of Radiology, UMDNJ-New Jersey Medical School,
Newark, NJ  - 973-97-6273

Dr. Lange declared chronic fatigue to be a 'brain disease'. Lange used functional neuroimaging to examine verbal working
memory in CFS patients and found dramatic differences in performance that could not be explained by mood or anxiety.
The neural networks in CFS patients, as measured by he blood oxygen signals, worked much harder than non-CFS patients
when given the same task to complete. Lange said this would explain the subjective reports of neuorocognitive difficulties in
information processing that are a common complaint among CFS patients.



Nancy Pederson, MD
Department of Medical Epidemiology and Biostatistics
Karolinska Institute, Stockholm, Sweden

Dr. Pederson reviewed telephone interview data from 12,407 sets of twins enrolled in the Swedish Twin Registry and found
that genetic factors in CFS were "modest", explaining only about 35% of the disease. This suggests to Pederson that
environmental triggers, such as toxins, stress and viruses, are important to the pathogenesis of CFS.



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